3BE7
Crystal structure of Zn-dependent arginine carboxypeptidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-08 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 113.420, 146.583, 255.963 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.22767 |
| Rwork | 0.226 |
| R-free | 0.27981 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2R8C |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.050 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.3.0034) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.130 | 0.520 |
| Number of reflections | 230541 | |
| <I/σ(I)> | 3.7 | 0.8 |
| Completeness [%] | 94.8 | 87.4 |
| Redundancy | 4.2 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 294 | 100mM Succinic acid pH 7.0, 15% PEG 3350, 10% Glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
