3BE4
Crystal structure of Cryptosporidium parvum adenylate kinase cgd5_3360
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97934 |
| Spacegroup name | H 3 |
| Unit cell lengths | 101.175, 101.175, 72.433 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.370 - 1.600 |
| R-factor | 0.164 |
| Rwork | 0.162 |
| R-free | 0.20200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ak2 |
| RMSD bond length | 0.035 |
| RMSD bond angle | 2.939 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.067 | 0.466 |
| Number of reflections | 36167 | |
| <I/σ(I)> | 11 | 3.35 |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 2.5 M Ammonium sulfate, 0.1 M BTP pH 7.0, 10 mM Adenosine-5-pentaphosphate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
