3BE4
Crystal structure of Cryptosporidium parvum adenylate kinase cgd5_3360
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-07-22 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97934 |
Spacegroup name | H 3 |
Unit cell lengths | 101.175, 101.175, 72.433 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.370 - 1.600 |
R-factor | 0.164 |
Rwork | 0.162 |
R-free | 0.20200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ak2 |
RMSD bond length | 0.035 |
RMSD bond angle | 2.939 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.067 | 0.466 |
Number of reflections | 36167 | |
<I/σ(I)> | 11 | 3.35 |
Completeness [%] | 99.0 | 100 |
Redundancy | 3.6 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 2.5 M Ammonium sulfate, 0.1 M BTP pH 7.0, 10 mM Adenosine-5-pentaphosphate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |