3BDU
Crystal structure of protein Q6D8G1 at the resolution 1.9 A. Northeast Structural Genomics Consortium target EwR22A.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4C |
Synchrotron site | NSLS |
Beamline | X4C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-10-03 |
Detector | MAR CCD 165 mm |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 105.974, 79.077, 48.472 |
Unit cell angles | 90.00, 96.30, 90.00 |
Refinement procedure
Resolution | 19.800 - 1.900 |
R-factor | 0.21 |
Rwork | 0.210 |
R-free | 0.24700 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.230 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SnB |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.069 | 0.358 |
Number of reflections | 61408 | |
<I/σ(I)> | 17.1 | 3.6 |
Completeness [%] | 99.8 | 98.8 |
Redundancy | 4.3 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.15 | 293 | 25% PEG 400, 0.1M Ca Acetate, 0.1M MES, pH 6.15, VAPOR DIFFUSION, SITTING DROP, temperature 293K |