3BDQ
Room Tempreture Crystal Structure of Sterol Carrier Protein-2 Like-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 297 |
| Detector technology | CCD |
| Collection date | 2006-07-04 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.250, 57.250, 49.100 |
| Unit cell angles | 90.00, 114.96, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.000 |
| R-factor | 0.188 |
| Rwork | 0.186 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.124 |
| Data reduction software | PROTEUM PLUS (PLUS) |
| Data scaling software | SAINT (V7.23A) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 14850 | |
| <I/σ(I)> | 21.24 | 4.97 |
| Completeness [%] | 93.7 | 91.4 |
| Redundancy | 4.93 | 2.22 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 297 | 2 microliters AeSCP-2L2 at 10 mg/ml was combined with 2 microliters 1.6 M sodium citrate, 5% glycerol, 100 mM Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
