3BDK
Crystal Structure of Streptococcus suis mannonate dehydratase complexed with substrate analogue
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 105.468, 105.468, 160.155 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.270 - 2.500 |
| R-factor | 0.25283 |
| Rwork | 0.250 |
| R-free | 0.30287 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vl8 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.450 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.063 | 0.509 |
| Number of reflections | 31751 | |
| <I/σ(I)> | 39.9 | 2.98 |
| Redundancy | 13.3 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | EVAPORATION |
