3BDE
Crystal structure of a dabb family protein with a ferredoxin-like fold (mll5499) from mesorhizobium loti maff303099 at 1.79 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9537, 0.9797, 0.9796 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 118.669, 28.415, 76.985 |
| Unit cell angles | 90.00, 117.78, 90.00 |
Refinement procedure
| Resolution | 29.540 - 1.790 |
| R-factor | 0.179 |
| Rwork | 0.177 |
| R-free | 0.22400 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.620 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.3.0040) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.540 | 29.540 | 1.840 |
| High resolution limit [Å] | 1.790 | 8.010 | 1.790 |
| Rmerge | 0.074 | 0.053 | 0.465 |
| Total number of observations | 593 | 1847 | |
| Number of reflections | 20638 | ||
| <I/σ(I)> | 7.8 | 8.8 | 1.7 |
| Completeness [%] | 94.5 | 93.9 | 73.2 |
| Redundancy | 2.3 | 2.2 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.9 | 277 | NANODROP, 0.2M Na Acetate, 20.0% PEG 3350, No Buffer pH 7.9, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
