3BDC
Crystal structure of Staphylococcal nuclease variant Delta+PHS at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2007-09-05 |
| Detector | BRUKER APEX II |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.097, 60.651, 36.948 |
| Unit cell angles | 90.00, 94.44, 90.00 |
Refinement procedure
| Resolution | 31.010 - 1.800 |
| R-factor | 0.19556 |
| Rwork | 0.190 |
| R-free | 0.25120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1TQO.PDB where all waters removed all Bs=20.0 A^2 and E92A |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.772 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.500 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 14206 | |
| <I/σ(I)> | 51.898 | 6.22 |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 13.18 | 7.86 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 17% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 8.0, vapor diffusion, hanging drop, temperature 277K |
