3BDA
Glycogen Phosphorylase complex with 1(-D-glucopyranosyl) cyanuric acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2005-10-02 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.8113 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 128.591, 128.591, 116.214 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.490 - 2.000 |
R-factor | 0.1851 |
Rwork | 0.185 |
R-free | 0.21050 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2prj |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 65903 | |
<I/σ(I)> | 9.78 | 3.57 |
Completeness [%] | 98.9 | 98.7 |
Redundancy | 4.3 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | SMALL TUBES | 6.7 | 289 | 10mM BES buffer, 3mM DDT, pH6.7, SMALL TUBES, temperature 289K |