3BD6
Glycogen Phosphorylase complex with 1(-D-ribofuranosyl) cyanuric acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2005-10-02 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.81 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 128.620, 128.620, 116.379 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 21.580 - 2.000 |
R-factor | 0.19092 |
Rwork | 0.190 |
R-free | 0.20515 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2prj |
RMSD bond length | 0.007 |
RMSD bond angle | 1.023 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 65282 | |
<I/σ(I)> | 18.6 | 4.5 |
Completeness [%] | 98.5 | 98.1 |
Redundancy | 4.9 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | SMALL TUBES | 6.7 | 289 | 10mM Bes buffer, 3mM DDT, pH6.7, SMALL TUBES, temperature 289K |