3BD1

Structure of the Cro protein from putative prophage element Xfaso 1 in Xylella fastidiosa strain Ann-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2005-11-05
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9002
Spacegroup name	P 32
Unit cell lengths	66.970, 66.970, 54.360
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	17.060 - 1.400
R-factor	0.147
Rwork	0.147
R-free	0.18200
Structure solution method	ACORN (DDM)
Starting model (for MR)	2hin
RMSD bond length	0.010
RMSD bond angle	1.792
Data reduction software	d*TREK
Data scaling software	d*TREK (9.2SSI)
Phasing software	ACORN
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	58.030	17.060	1.450
High resolution limit [Å]	1.400	3.010	1.400
Rmerge	0.048	0.025	0.386
Total number of observations		15717	19627
Number of reflections	53726
<I/σ(I)>	13.3	44.3	3.2
Completeness [%]	98.6	87.2	99.7
Redundancy	3.61	3.25	3.65

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	16% glycerol, 1.5 M ammonium sulfate, 0.1 M Tris, 1 mM TCEP-hydrochloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K