3BD0
Crystal structure of Memo, form II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Detector technology | CCD |
Collection date | 2007-10-20 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97989 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 139.877, 88.307, 97.051 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.680 - 3.010 |
R-factor | 0.217 |
Rwork | 0.213 |
R-free | 0.28000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3bcz |
RMSD bond length | 0.010 |
RMSD bond angle | 1.330 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 3.110 |
High resolution limit [Å] | 3.000 | 6.440 | 3.000 |
Rmerge | 0.227 | 0.084 | 0.626 |
Number of reflections | 24559 | ||
<I/σ(I)> | 3.5 | ||
Completeness [%] | 99.9 | 99.7 | 99.8 |
Redundancy | 7.2 | 6.8 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | Crystal was grown in 22.5% PEG 3350, 0.1 M MES pH 5.5. Soaked in 5 mM ZnCl2, 22.5% PEG 3350 pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |