3BCZ
Crystal structure of Memo
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97990 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 139.826, 88.849, 98.089 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.970 - 2.100 |
| R-factor | 0.18537 |
| Rwork | 0.183 |
| R-free | 0.23005 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.536 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 4.520 | 2.100 |
| Rmerge | 0.136 | 0.072 | 0.606 |
| Number of reflections | 71140 | ||
| <I/σ(I)> | 7.9 | ||
| Completeness [%] | 99.0 | 99.7 | 98.2 |
| Redundancy | 6.6 | 6.3 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 22.5% PEG 3350, 0.1 M MES pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
