Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2007-02-15 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.8 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 128.436, 128.436, 116.310 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.730 - 2.140 |
R-factor | 0.18516 |
Rwork | 0.183 |
R-free | 0.21882 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2prj |
RMSD bond length | 0.007 |
RMSD bond angle | 1.044 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.180 |
High resolution limit [Å] | 2.140 | 2.140 |
Number of reflections | 53628 | |
<I/σ(I)> | 17.2 | 4.7 |
Completeness [%] | 99.0 | 98.6 |
Redundancy | 6.6 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | SMALL TUBES | 6.7 | 289 | 10mM Bes buffer, 3mM DDT, pH6.7, SMALL TUBES, temperature 289K |