3BCM
Crystal Structure of The Unswapped Form of P19A/L28Q/N67D BS-RNase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2004-11-22 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.513, 59.508, 49.738 |
Unit cell angles | 90.00, 118.02, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.250 |
R-factor | 0.183 |
Rwork | 0.183 |
R-free | 0.25200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1r5d |
RMSD bond length | 0.020 |
RMSD bond angle | 2.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.320 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.070 | 0.129 |
Number of reflections | 11915 | |
<I/σ(I)> | 22 | 5 |
Completeness [%] | 99.5 | 97.5 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.4 | 277 | 30% methyl pentanediol, 50mM TRIS-HCl pH 8.4, 0.1M ammonium phosphate, VAPOR DIFFUSION, SITTING DROP, temperature 277K |