3BCG
Conformational changes of the AcrR regulator reveal a mechanism of induction
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-13 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 31 |
| Unit cell lengths | 46.660, 46.660, 166.155 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.480 |
| Rwork | 0.214 |
| R-free | 0.26800 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SnB |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.570 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Rmerge | 0.051 | 0.246 |
| Number of reflections | 14362 | |
| <I/σ(I)> | 23.2 | 4 |
| Completeness [%] | 84.5 | |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 35% PEG4000, 0.2M MgCl2, 0.1M Tris , pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
