3BCF
Alpha-amylase B from Halothermothrix orenii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-01 |
| Detector | SBC |
| Wavelength(s) | 0.978 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 226.620, 77.820, 50.330 |
| Unit cell angles | 90.00, 99.38, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.1927 |
| Rwork | 0.191 |
| R-free | 0.22869 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vjs |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.796 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 51305 | |
| <I/σ(I)> | 9.5 | 2.7 |
| Completeness [%] | 98.3 | 99.4 |
| Redundancy | 4.7 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 30% (w/v) monomethylether PEG 5000, 0.1M Mes-OH, 0.2M ammonium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
