3BCE

Crystal structure of the ErbB4 kinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4C
Synchrotron site	NSLS
Beamline	X4C
Detector technology	IMAGE PLATE
Collection date	2007-08-25
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.97893
Spacegroup name	P 32
Unit cell lengths	86.723, 86.723, 120.008
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.500
R-factor	0.198
Rwork	0.195
R-free	0.25100
RMSD bond length	0.010
RMSD bond angle	1.297
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.590
High resolution limit [Å]	2.500	5.380	2.500
Rmerge	0.107	0.049	0.457
Number of reflections	34855
<I/σ(I)>	6.1
Completeness [%]	100.0	100	100
Redundancy	2.6	2.6	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	15% PEG3350, 0.1 M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K