3BCB

Crystal structure of mouse selenocysteine synthase, sodium phosphate soak

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	OTHER
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2007-10-04
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54179
Spacegroup name	I 2 2 2
Unit cell lengths	59.231, 138.974, 141.821
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.850
R-factor	0.16784
Rwork	0.166
R-free	0.20265
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bc8
RMSD bond length	0.010
RMSD bond angle	1.193
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.950
High resolution limit [Å]	1.850	1.850
Number of reflections	49649
<I/σ(I)>	16.3	1.9
Completeness [%]	99.3	99.2
Redundancy	3.5	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	11 % Ethylene glycol, soaked in 0.5 M Na phosphate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K