3BBZ

Structure of the nucleocapsid-binding domain from the mumps virus phosphoprotein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-12-02
Detector	ADSC QUANTUM 315
Wavelength(s)	0.920
Spacegroup name	P 21 21 21
Unit cell lengths	23.992, 55.485, 60.019
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.740 - 2.100
R-factor	0.19254
Rwork	0.190
R-free	0.24110
Structure solution method	MAD
RMSD bond length	0.020
RMSD bond angle	1.606
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.270	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.140	0.343
Number of reflections	4915
<I/σ(I)>	5.8	2.4
Completeness [%]	97.2	98.6
Redundancy	8.4	8.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.7	277	0.2M Alanine/KOH pH 9.7, 3.6-4.6M Ammonium formate, VAPOR DIFFUSION, SITTING DROP, temperature 277K
1	VAPOR DIFFUSION, SITTING DROP	9.7	277	0.2M Alanine/KOH pH 9.7, 3.6-4.6M Ammonium formate, VAPOR DIFFUSION, SITTING DROP, temperature 277K