3BBW
crystal structure of the ErbB4 kinase in its inactive conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CAMD BEAMLINE GCPCC |
| Synchrotron site | CAMD |
| Beamline | GCPCC |
| Detector technology | CCD |
| Collection date | 2007-05-08 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | P 61 |
| Unit cell lengths | 102.746, 102.746, 181.335 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 4.000 |
| R-factor | 0.305 |
| Rwork | 0.302 |
| R-free | 0.35400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.639 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 4.140 |
| High resolution limit [Å] | 4.000 | 8.560 | 4.000 |
| Rmerge | 0.253 | 0.076 | 0.667 |
| Number of reflections | 9061 | ||
| <I/σ(I)> | 4.2 | ||
| Completeness [%] | 98.6 | 100 | 93.3 |
| Redundancy | 7.5 | 10 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 2.2 M NaCl, 0.2 M MgCl2, 0.1 M hepes, pH 7.0, 2% hexafluoroisopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
