3BBL
Crystal structure of a regulatory protein of LacI family from Chloroflexus aggregans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97900 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 122.550, 122.550, 123.301 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.380 - 2.350 |
| R-factor | 0.241 |
| Rwork | 0.240 |
| R-free | 0.25800 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Number of reflections | 22738 | |
| <I/σ(I)> | 12.7 | 2 |
| Completeness [%] | 96.7 | 79 |
| Redundancy | 21.4 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 5% PEG 2000, 40% Tacsimate, 5% Ethylene glycol, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
