3BBK
Miminally Junctioned Hairpin Ribozyme Incorporates A38C and 2'5'-phosphodiester Linkage within Active Site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-05-10 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.918 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 93.520, 93.520, 133.970 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.750 |
R-factor | 0.197 |
Rwork | 0.195 |
R-free | 0.22100 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2p7f |
RMSD bond length | 0.009 |
RMSD bond angle | 1.500 |
Data reduction software | CrystalClear |
Data scaling software | CrystalClear |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.950 | 2.850 |
High resolution limit [Å] | 2.750 | 2.750 |
Number of reflections | 9471 | |
<I/σ(I)> | 34.4 | 4 |
Completeness [%] | 99.6 | 99.9 |
Redundancy | 13.8 | 10.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | PEG 2K MME, lithium sulfate, sodium cacodylate, spermidine, cobalt hexaammine, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 2K MME | ||
2 | 1 | 1 | Li2SO4 | ||
3 | 1 | 1 | sodium cacodylate | ||
4 | 1 | 1 | spermidine | ||
5 | 1 | 1 | cobalt hexaammine | ||
6 | 1 | 2 | PEG 2K MME | ||
7 | 1 | 2 | Li2SO4 | ||
8 | 1 | 2 | sodium cacodylate | ||
9 | 1 | 2 | spermidine |