3BBK
Miminally Junctioned Hairpin Ribozyme Incorporates A38C and 2'5'-phosphodiester Linkage within Active Site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-10 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 93.520, 93.520, 133.970 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.750 |
| R-factor | 0.197 |
| Rwork | 0.195 |
| R-free | 0.22100 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2p7f |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.950 | 2.850 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Number of reflections | 9471 | |
| <I/σ(I)> | 34.4 | 4 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 13.8 | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | PEG 2K MME, lithium sulfate, sodium cacodylate, spermidine, cobalt hexaammine, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 2K MME | ||
| 2 | 1 | 1 | Li2SO4 | ||
| 3 | 1 | 1 | sodium cacodylate | ||
| 4 | 1 | 1 | spermidine | ||
| 5 | 1 | 1 | cobalt hexaammine | ||
| 6 | 1 | 2 | PEG 2K MME | ||
| 7 | 1 | 2 | Li2SO4 | ||
| 8 | 1 | 2 | sodium cacodylate | ||
| 9 | 1 | 2 | spermidine |
