3BBC
Crystal structure of R88A mutant of the NM23-H2 transcription factor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-12-13 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 71.756, 104.616, 118.089 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.500 - 1.700 |
R-factor | 0.17589 |
Rwork | 0.174 |
R-free | 0.21574 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1nue |
RMSD bond length | 0.019 |
RMSD bond angle | 1.720 |
Data reduction software | CrystalClear |
Data scaling software | CrystalClear |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.500 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.093 | 0.430 |
Number of reflections | 98113 | |
<I/σ(I)> | 16.9 | 3.4 |
Completeness [%] | 99.8 | 99.4 |
Redundancy | 7.2 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | PEG 1500, 50 mM sodium citrate, 20 mM MgCl2, 5 mM DTT, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |