3BB4
Crystal structure of Toc33 from Arabidopsis thaliana in complex with Mg2+ and GMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9746 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 121.567, 121.567, 42.879 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.850 |
| R-factor | 0.22082 |
| Rwork | 0.218 |
| R-free | 0.27663 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h65 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.073 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.3.0008) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.940 |
| High resolution limit [Å] | 2.840 | 2.840 |
| Rmerge | 0.085 | 0.486 |
| Number of reflections | 7827 | |
| <I/σ(I)> | 15.39 | 2.2 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 5.8 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 296 | 22% PEG1500, 15% glycerol, pH7.4, VAPOR DIFFUSION, SITTING DROP, temperature 296K |
