3BB3
Crystal structure of Toc33 from Arabidopsis thaliana in complex with GDP and Mg2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0723 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 121.617, 121.617, 42.769 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.940 |
| R-factor | 0.22006 |
| Rwork | 0.218 |
| R-free | 0.26196 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h65 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.057 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.3.0008) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.000 | 3.040 |
| High resolution limit [Å] | 2.900 | 2.940 |
| Rmerge | 0.081 | 0.466 |
| Number of reflections | 7234 | |
| <I/σ(I)> | 14.4 | 1.9 |
| Completeness [%] | 99.3 | 97.9 |
| Redundancy | 6.1 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 292 | 24% PEG1500, 20% Glycerol, pH7.4, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
