3BB1
Crystal structure of Toc34 from Pisum sativum in complex with Mg2+ and GMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0723 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 178.776, 180.061, 90.948 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.880 - 2.800 |
| R-factor | 0.22714 |
| Rwork | 0.224 |
| R-free | 0.28231 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h65 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.055 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.3.0008) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 72688 | |
| <I/σ(I)> | 15.5 | 2.4 |
| Completeness [%] | 99.5 | 95.9 |
| Redundancy | 3.8 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 296 | 0.2M di-potassiumphosphate 20% PEG3350, pH7.4, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
