3BAZ
Structure of hydroxyphenylpyruvate reductase from coleus blumei in complex with NADP+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-10-10 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.54178 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 63.500, 63.500, 222.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.200 |
R-factor | 0.2107 |
Rwork | 0.211 |
R-free | 0.26030 |
Structure solution method | AB INITIO |
Starting model (for MR) | 3ba1 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.020 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.250 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 23761 | |
<I/σ(I)> | 24.3 | 2.6 |
Completeness [%] | 98.1 | 81.4 |
Redundancy | 12.3 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 299 | 20% PEG1000, 0.1M Imidazol, pH7.5, VAPOR DIFFUSION, SITTING DROP, temperature 299K |