3BAU
Crystal structure of K12V/L26D/D28A mutant of Human acidic fibroblast growth factor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 103 |
Detector technology | CCD |
Collection date | 2006-10-11 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 75.462, 96.793, 108.010 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.590 - 1.600 |
R-factor | 0.205 |
Rwork | 0.205 |
R-free | 0.23300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jqz |
RMSD bond length | 0.013 |
RMSD bond angle | 1.600 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.590 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.077 | 0.310 |
Number of reflections | 43178 | |
<I/σ(I)> | 32.16 | 3.95 |
Completeness [%] | 87.5 | 99.8 |
Redundancy | 5.6 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298.15 | 2.7M Na-formate, 0.7M (NH4)2SO4, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.15K |