3BA1
Structure of hydroxyphenylpyruvate reductase from coleus blumei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-28 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.91838 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 62.940, 62.940, 153.256 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 1.470 |
| Rwork | 0.149 |
| R-free | 0.20310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.029 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.500 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Number of reflections | 58791 | |
| <I/σ(I)> | 23.9 | 3.4 |
| Completeness [%] | 96.9 | 99.1 |
| Redundancy | 5.8 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | MPD, SODIUM CHLORIDE, pH7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
