3B9Z
Crystal structure of the Nitrosomonas europaea Rh protein complexed with carbon dioxide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.91837 |
| Spacegroup name | H 3 |
| Unit cell lengths | 100.434, 100.434, 143.467 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.631 - 1.850 |
| R-factor | 0.166 |
| Rwork | 0.166 |
| R-free | 0.17900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3b9y |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.002 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.631 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.076 | 0.523 |
| Number of reflections | 45094 | |
| <I/σ(I)> | 7.48 | 1.6 |
| Completeness [%] | 97.9 | 95.9 |
| Redundancy | 3.4 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 14.5% PEG 2000 MME, 1 mM EDTA, 0.1 M MES buffer, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
