3B9Y

Crystal structure of the Nitrosomonas europaea Rh protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-05-03
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.9794
Spacegroup name	H 3
Unit cell lengths	100.438, 100.438, 143.875
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.631 - 1.850
R-factor	0.161
Rwork	0.160
R-free	0.18300
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.982
Data reduction software	MOSFLM
Data scaling software	SCALEPACK
Phasing software	SHELXS
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.631	1.900
High resolution limit [Å]	1.850	1.850
Rmerge	0.094	0.441
Number of reflections	46149
<I/σ(I)>	7	2.2
Completeness [%]	99.9	99.3
Redundancy	3.5	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	14.5% PEG 2000 MME, 1 mM EDTA, 0.1 M MES buffer, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K