3B9W
The 1.3 A resolution structure of Nitrosomonas europaea Rh50 and mechanistic implications for NH3 transport by Rhesus family proteins
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-26 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.07209 |
| Spacegroup name | H 3 |
| Unit cell lengths | 100.061, 100.061, 143.828 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.300 |
| R-factor | 0.14809 |
| Rwork | 0.147 |
| R-free | 0.17082 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB Entrty 2NUU |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.005 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.400 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Number of reflections | 131874 | |
| <I/σ(I)> | 18.71 | |
| Completeness [%] | 99.8 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 10mM Tris/HCl pH=8.5, 0.2M NaCl, 24% PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
