3B9W
The 1.3 A resolution structure of Nitrosomonas europaea Rh50 and mechanistic implications for NH3 transport by Rhesus family proteins
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-03-26 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.07209 |
Spacegroup name | H 3 |
Unit cell lengths | 100.061, 100.061, 143.828 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.300 |
R-factor | 0.14809 |
Rwork | 0.147 |
R-free | 0.17082 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB Entrty 2NUU |
RMSD bond length | 0.006 |
RMSD bond angle | 1.005 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.400 |
High resolution limit [Å] | 1.300 | 1.300 |
Number of reflections | 131874 | |
<I/σ(I)> | 18.71 | |
Completeness [%] | 99.8 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 10mM Tris/HCl pH=8.5, 0.2M NaCl, 24% PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K |