3B9M

Human serum albumin complexed with myristate, 3'-azido-3'-deoxythymidine (AZT) and salicylic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2006-08-22
Spacegroup name	C 1 2 1
Unit cell lengths	181.536, 38.458, 95.368
Unit cell angles	90.00, 104.92, 90.00

Refinement procedure

Resolution	46.080 - 2.700
Rwork	0.242
R-free	0.31600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1e7g
RMSD bond length	0.011
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.000	2.870
High resolution limit [Å]	2.700	2.700
Number of reflections	15958
Completeness [%]	54.0
Redundancy	6	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	32%(w/v) polyethylene glycol 3350, 50mM potassium phosphate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K