3B9C
Crystal Structure of Human GRP CRD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 3W1A |
| Synchrotron site | BSRF |
| Beamline | 3W1A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-21 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 123.140, 96.670, 61.570 |
| Unit cell angles | 90.00, 118.79, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.22357 |
| Rwork | 0.221 |
| R-free | 0.26631 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x50 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.533 |
| Data reduction software | AUTOMAR |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.870 |
| High resolution limit [Å] | 1.810 | 1.810 |
| Rmerge | 0.065 | 0.275 |
| Number of reflections | 49473 | |
| <I/σ(I)> | 4.3 | 1 |
| Completeness [%] | 93.9 | 80.8 |
| Redundancy | 3.4 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 100mM Tris-HCl pH7.5, 2% PEG 400, 2M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
