3B93
crystal structure of human GITRL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-07-06 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 104.413, 46.384, 76.460 |
| Unit cell angles | 90.00, 91.56, 90.00 |
Refinement procedure
| Resolution | 75.000 - 2.200 |
| R-factor | 0.20872 |
| Rwork | 0.204 |
| R-free | 0.27992 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | mouse GITRL |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.521 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.080 | 0.547 |
| Number of reflections | 16198 | |
| <I/σ(I)> | 127.9 | 5.8 |
| Completeness [%] | 96.9 | 87.8 |
| Redundancy | 5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 0.2M Lithium sulfate, 0.1M Tris, 30% PEG4000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
