3B91
Minimally Hinged Hairpin Ribozyme Incorporates Ade38(2AP) and 2',5'-Phosphodiester Linkage Mutations at the Active Site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-10 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 94.030, 94.030, 134.290 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.040 - 2.750 |
| R-factor | 0.218 |
| Rwork | 0.216 |
| R-free | 0.24300 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2p7f |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.040 | 2.850 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Number of reflections | 9589 | |
| <I/σ(I)> | 23.2 | 3 |
| Completeness [%] | 99.5 | 99.4 |
| Redundancy | 9.8 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | PEG 2K MME, lithium sulfate, sodium cacodylate, spermidine, cobalt hexaammine, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 2K MME | ||
| 2 | 1 | 1 | Li2SO4 | ||
| 3 | 1 | 1 | sodium cacodylate | ||
| 4 | 1 | 1 | spermidine | ||
| 5 | 1 | 1 | cobalt hexaammine | ||
| 6 | 1 | 2 | PEG 2K MME | ||
| 7 | 1 | 2 | Li2SO4 | ||
| 8 | 1 | 2 | spermidine |
