3B8N
Structure of FepE- Bacterial Polysaccharide Co-polymerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 160.670, 160.670, 276.938 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.100 |
| Rwork | 0.254 |
| R-free | 0.28200 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.396 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (refmac_5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 6.400 | 3.000 |
| Rmerge | 0.078 | 0.037 | 0.365 |
| Number of reflections | 83083 | ||
| <I/σ(I)> | 9.8 | ||
| Completeness [%] | 99.8 | 98.6 | 100 |
| Redundancy | 8.3 | 8.7 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 298 | Sodium Citrate Na3C6H5O7, pH 7.5, vapor diffusion, temperature 298K |
