3B8M
Structure of FepE- Bacterial Polysaccharide Co-polymerase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 139.726, 139.726, 276.654 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.130 - 2.700 |
R-factor | 0.22803 |
Rwork | 0.226 |
R-free | 0.26947 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.367 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (refmac_5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.087 | 0.448 |
Number of reflections | 44193 | |
<I/σ(I)> | 10.3 | |
Completeness [%] | 99.1 | 92.2 |
Redundancy | 11.8 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 298 | Sodium Citrate Na3C6H5O7, pH 7.5, vapor diffusion, temperature 298K, pH 7.50 |