3B8M
Structure of FepE- Bacterial Polysaccharide Co-polymerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 139.726, 139.726, 276.654 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.130 - 2.700 |
| R-factor | 0.22803 |
| Rwork | 0.226 |
| R-free | 0.26947 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.367 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (refmac_5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.087 | 0.448 |
| Number of reflections | 44193 | |
| <I/σ(I)> | 10.3 | |
| Completeness [%] | 99.1 | 92.2 |
| Redundancy | 11.8 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 298 | Sodium Citrate Na3C6H5O7, pH 7.5, vapor diffusion, temperature 298K, pH 7.50 |
