3B8H

Structure of the eEF2-ExoA(E546A)-NAD+ complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2006-07-09
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0000
Spacegroup name	C 1 2 1
Unit cell lengths	329.440, 68.160, 191.630
Unit cell angles	90.00, 103.28, 90.00

Refinement procedure

Resolution	24.992 - 2.500
R-factor	0.214
Rwork	0.214
R-free	0.25600
Starting model (for MR)	2b82
RMSD bond length	0.002
RMSD bond angle	0.637
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.138	0.683
Number of reflections	143628	15781
<I/σ(I)>	9.79	2.1
Completeness [%]	99.6	99.7
Redundancy	4.1	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.2	293	7% PEG-10000, 3.5mM MPD, 100 mM HEPES, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K