3B8F
Crystal structure of the cytidine deaminase from Bacillus anthracis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.078, 98.479, 61.360 |
| Unit cell angles | 90.00, 111.09, 90.00 |
Refinement procedure
| Resolution | 57.260 - 1.900 |
| R-factor | 0.16908 |
| Rwork | 0.168 |
| R-free | 0.19589 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.364 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.260 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.092 | 0.683 |
| Number of reflections | 45458 | |
| <I/σ(I)> | 22.2 | 1.43 |
| Completeness [%] | 96.2 | 83.56 |
| Redundancy | 4.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.1M HEPES, 1.4M Tri-sodium citrate dihydrate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
