3B7G
Human DEAD-box RNA helicase DDX20, Conserved domain I (DEAD) in complex with AMPPNP (Adenosine-(Beta,gamma)-imidotriphosphate)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00595 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 63.560, 63.560, 214.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.430 - 1.900 |
| R-factor | 0.17368 |
| Rwork | 0.172 |
| R-free | 0.20219 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oxc |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.648 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.3.0040) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.081 | 0.126 |
| Number of reflections | 40642 | |
| <I/σ(I)> | 32.1 | 21.3 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 20.5 | 18.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 25% PEG 3350, 200mM Ammonium acetate, 100mM Bis-Tris, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
