3B7F

Crystal structure of a putative glycosyl hydrolase with bnr repeats (reut_b4987) from ralstonia eutropha jmp134 at 2.20 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-10-04
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9537, 0.9796, 0.9798
Spacegroup name	I 41 2 2
Unit cell lengths	114.310, 114.310, 161.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.583 - 2.200
R-factor	0.17
Rwork	0.168
R-free	0.21600
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.859
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.583	28.583	2.280
High resolution limit [Å]	2.200	4.720	2.200
Rmerge	0.075	0.025	0.509
Number of reflections	27218	5016	4692
<I/σ(I)>	9.66	25.4	1.7
Completeness [%]	93.0	94.9	88.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	277	NANODROP, 1.0M LiCl, 10.0% PEG 6000, 0.1M Tris-HCl pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K