3B7F
Crystal structure of a putative glycosyl hydrolase with bnr repeats (reut_b4987) from ralstonia eutropha jmp134 at 2.20 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-10-04 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9537, 0.9796, 0.9798 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 114.310, 114.310, 161.430 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.583 - 2.200 |
R-factor | 0.17 |
Rwork | 0.168 |
R-free | 0.21600 |
Structure solution method | MAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.859 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELX |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 28.583 | 28.583 | 2.280 |
High resolution limit [Å] | 2.200 | 4.720 | 2.200 |
Rmerge | 0.075 | 0.025 | 0.509 |
Number of reflections | 27218 | 5016 | 4692 |
<I/σ(I)> | 9.66 | 25.4 | 1.7 |
Completeness [%] | 93.0 | 94.9 | 88.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | NANODROP, 1.0M LiCl, 10.0% PEG 6000, 0.1M Tris-HCl pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |