3B77

Crystal structure of a ph domain containing bacterial protein (exig_2160) from exiguobacterium sibiricum 255-15 at 2.42 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-08-31
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9799, 0.9795, 1.0000
Spacegroup name	P 4
Unit cell lengths	150.990, 150.990, 76.219
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.727 - 2.420
R-factor	0.216
Rwork	0.214
R-free	0.25400
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.498
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.727	47.727	2.510
High resolution limit [Å]	2.420	5.190	2.420
Rmerge	0.081	0.033	0.787
Number of reflections	65515	6750	6759
<I/σ(I)>	11.02	26.9	1.9
Completeness [%]	99.7	98.5	99.9
Redundancy	3.71		3.74

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	277	NANODROP, 10.0% PEG 6000, 0.1M Bicine pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K