3B75
Crystal Structure of Glycated Human Haemoglobin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2003-01-01 |
| Wavelength(s) | 0.95400 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 237.987, 59.267, 137.024 |
| Unit cell angles | 90.00, 125.36, 90.00 |
Refinement procedure
| Resolution | 14.900 - 2.300 |
| R-factor | 0.249 |
| Rwork | 0.244 |
| R-free | 0.33100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hho |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.755 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.034 | 0.391 |
| Number of reflections | 64184 | |
| <I/σ(I)> | 23.7 | 2.5 |
| Completeness [%] | 92.3 | 71 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 277 | PEG 4000, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
