3B6Y
Crystal Structure of the Second HIN-200 Domain of Interferon-Inducible Protein 16
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-06-24 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.97800 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.999, 92.887, 100.372 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.140 - 2.350 |
R-factor | 0.229 |
Rwork | 0.226 |
R-free | 0.29200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2oq0 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.466 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (refmac_5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.430 |
High resolution limit [Å] | 2.350 | 5.060 | 2.350 |
Rmerge | 0.066 | 0.048 | 0.379 |
Number of reflections | 17421 | ||
<I/σ(I)> | 12.4 | ||
Completeness [%] | 99.9 | 99.5 | 100 |
Redundancy | 7.2 | 6.6 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 30% PEG 4000, 0.2M Ammonium sulfate, 0.1M Sodium cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |