3B6U
Crystal structure of the motor domain of human kinesin family member 3B in complex with ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-08-08 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.731, 95.852, 63.883 |
Unit cell angles | 90.00, 92.61, 90.00 |
Refinement procedure
Resolution | 19.860 - 1.800 |
R-factor | 0.192 |
Rwork | 0.191 |
R-free | 0.23300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1goj |
RMSD bond length | 0.016 |
RMSD bond angle | 1.458 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (refmac_5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 20.000 | 1.860 |
High resolution limit [Å] | 1.800 | 3.870 | 1.800 |
Rmerge | 0.050 | 0.028 | 0.377 |
Number of reflections | 64524 | ||
<I/σ(I)> | 17.1 | ||
Completeness [%] | 98.9 | 99.9 | 96.7 |
Redundancy | 3.6 | 3.7 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 291 | 30% PEG 3350, 0.1 M Tris-HCl, 0.2M Lithium sulfate, pH 8.5, VAPOR DIFFUSION, temperature 291K |