3B64
Macrophage Migration Inhibitory Factor (MIF) From /Leishmania Major
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.95 |
| Spacegroup name | H 3 |
| Unit cell lengths | 52.321, 52.321, 96.817 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 1.030 |
| R-factor | 0.1386 |
| Rwork | 0.139 |
| R-free | 0.15680 |
| Structure solution method | AB INITIO |
| RMSD bond length | 0.016 |
| RMSD bond angle | 0.030 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.070 |
| High resolution limit [Å] | 1.030 | 1.030 |
| Rmerge | 0.051 | 0.228 |
| Number of reflections | 46731 | |
| <I/σ(I)> | 22 | 6.8 |
| Completeness [%] | 95.5 | 71.6 |
| Redundancy | 5.2 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M HEPES-Na, 20% (v/v) iso-Propano polyethylene glycol 4000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
