3B5Z
Crystal Structure of MsbA from Salmonella typhimurium with ADP Vanadate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-09-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 267.169, 121.103, 176.744 |
| Unit cell angles | 90.00, 121.57, 90.00 |
Refinement procedure
| Resolution | 20.000 - 4.200 |
| Rwork | 0.336 |
| R-free | 0.36200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 4.460 |
| High resolution limit [Å] | 4.200 | 4.200 |
| Number of reflections | 30164 | |
| Completeness [%] | 86.3 | 73.6 |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.7 | 277 | 100mM NaCl, 325mM NaCH3COO, 19% PEG 400, 15mM ATP, 20mM MgCl2, 1mM sodium ortho-vanadate and 0.05% beta-UDM, pH 7.7, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
