3B5T

Crystal Structure of Novel Immune-Type Receptor 10 Se-Met Extracellular Fragment Mutant N30D

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-10-08
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9537
Spacegroup name	P 31 2 1
Unit cell lengths	90.305, 90.305, 137.402
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 1.750
R-factor	0.185
Rwork	0.185
R-free	0.21700
Structure solution method	SAD
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.760
High resolution limit [Å]	1.750	1.750
Rmerge	0.057	0.383
Number of reflections	66060
<I/σ(I)>	10.5
Completeness [%]	100.0	100
Redundancy	7.2	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	1.6M Na Citrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K