3B5S
Minimally Hinged Hairpin Ribozyme Incorporates A38DAP Mutation and 2'-O-methyl Modification at the Active Site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-09-30 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.977 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 93.500, 93.500, 133.420 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.490 - 2.250 |
R-factor | 0.223 |
Rwork | 0.223 |
R-free | 0.24100 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2oue |
RMSD bond length | 0.009 |
RMSD bond angle | 1.600 |
Data reduction software | CrystalClear |
Data scaling software | CrystalClear |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.490 | 2.350 |
High resolution limit [Å] | 2.250 | 2.250 |
Number of reflections | 16724 | |
<I/σ(I)> | 25.5 | 3.5 |
Completeness [%] | 98.5 | 98.1 |
Redundancy | 7.8 | 8.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | PEG 2K MME, lithium sulfate, sodium cacodylate, spermidine, cobalt hexaammine, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 2K MME | ||
2 | 1 | 1 | Li2SO4 | ||
3 | 1 | 1 | sodium cacodylate | ||
4 | 1 | 1 | spermidine | ||
5 | 1 | 1 | cobalt hexaammine | ||
6 | 1 | 2 | PEG 2K MME | ||
7 | 1 | 2 | Li2SO4 | ||
8 | 1 | 2 | spermidine |